Wilkinson

Our studies are centered on two topics:  homeobox gene circuits and RNA surveillance.  With regard to the first topic, we are investigating the function and regulation of the Rhox genes, a large set of homeobox genes clustered together in a single region of the X chromosome that encode transcription factors selectively expressed during embryogenesis and in adult reproductive organs.  Using knockout, tissue-specific knockdown, and genome-wide molecular approaches, we are elucidating their biological functions and gene targets in embryonic stem cells, spermatogonial stem cells, and adult reproductive organs.  To determine their place in transcriptional networks, we are elucidating the mechanisms that control their expression.  Epigenetic mechanisms are the focus of our studies.  Our second major interest is nonsense-mediated decay (NMD), a RNA surveillance pathway that rapidly degrades aberrant mRNAs and ~5% of normal mRNAs.  Our long-term interest is to understand the underlying molecular mechanism of NMD.  More recently, our studies have expanded to examine how NMD is regulated by both microRNAs and feedback networks.  We are also determining the physiological roles of NMD in mice in vivo.