Laurent Laboratory the Department of Reproductive Medicine

Louise Laurent, M.D., Ph.D.

Assistant Professor 
UCSD Dept of Reproductive Medicine
Division of Maternal Fetal Medicine


Education

  University of Chicago Laboratory High School 1983-1987
A.B. in Biochemistry Harvard College 1987-1990
Ph.D. in Biochemistry awarded in 1998
M.D. awarded in 2001
UCSF.  Medical Scientist Training Program 1990-2001
Residency in Reproductive Medicine UCSD 2001-2005
Fellowship in Maternal Fetal Medicine UCSD 2005-2008

Professional Positions

Assistant Adjunct Professor
Department: Reproductive Medicine. 
Chairman:  Dr. Thomas R. Moore.
UCSD 2008-2013
Assistant Professor, tenure track
Department: Reproductive Medicine.           
Chairman:  Dr. Thomas R. Moore.
UCSD 2013-present

Academic Honors and Awards

Chicago Museum of Science and Industry's Outstanding Young Scientist 1986
Harvard National Scholar 1987
Presidential Scholar 1987
Westinghouse Science Talent Search.  First Place Award 1987
Summa Cum Laude and Harvard Hoopes Award for Outstanding Senior Thesis in Biochemistry. 1990
Third Year Resident Research Award, University of California, San Diego 2004
California Institute for Regenerative Medicine Clinical Fellow Training Award 2006
California Institute for Regenerative Medicine Junior Investigator Travel Award 2007

Hospital Appointments

University of California San Diego Medical Center 2001-present
Scripps Mercy Hospital, San Diego, CA 2005-present
Scripps Memorial Hospital, La Jolla, CA 2006-present
Sharp Grossmont Hospital, San Diego, CA 2007-present
Palomar Hospital, Escondido, CA 2007-present
Pomerado Hospital, Poway, CA 2007-present
Scripps Encinitas Hospital, Encinitas, CA 2010-present

Medical Licensure and Certification

California Medical Board 2002-Present
American Board of Obstetrics and Gynecology, Board Certified in General Obstetrics and Gynecology 2007
American Board of Obstetrics and Gynecology, Board Certified in Maternal Fetal Medicine 2013

Professional Societies

American College of Obstetrics and Gynecology 2001-Present
Society for Maternal Fetal Medicine 2005-present
Society for Gynecologic Investigation 2007-present
International Society for Stem Cell Research 2007-present
Perinatal Research Society 2011-present

 

Peer Reviewed Research Articles

Ma H, Morey R, O’Neil RC, He Y, Daughtry B, Schultz MD, Hariharan M, Nery JR, Castanon R, Sabatini K, Thiagarajan RD, Tachibana M, Kang E, Tippner-Hedges R, Ahmed R, Gutierrez NM, Van Dyken C, Polat A, Sugawara A, Sparman M, Gokhale S, Amato P, Wolf DP, Ecker JR*, Laurent LC*, Mitalipov S*. 2014. “Abnormalities in human pluripotent cells due to reprogramming mechanisms.” Nature, Epub ahead of print. *Co-corresponding authors.
  Wolfe LM, Thiagarajan RD, Boscolo F, Tache V, Coleman RL, Kim J, Kwan WK, Loring JF, Parast M, Laurent LC. 2014. “Banking Placental Tissue: An Optimized Collection Procedure for Genome-Wide Analysis of Nucleic Acids.” Placenta, Epub ahead of print.
Ben-Yosef D, Boscolo FS, Amir H, Malcov M, Amit A, Laurent LC. 2013. “Genomic Analysis of hESC Pedigrees Identifies De Novo Mutations and Enables Determination of the Timing and Origin of Mutational Events.” Cell Reports, 4(6):1-15.
Li Y, Moretto-Zita M, Soncin F, Wakeland A, Wolfe L, Leon-Garcia S, Pandian R, Pizzo D, Cui L, Nazor K, Loring JF, Crum CP, Laurent LC, Parast MM. 2013. “BMP4-directed trophoblast differentiation of human embryonic stem cells is mediated through a DNp63+ cytotrophoblast stem cell state.” Development, 140(19):3965-76.
Liao X, Xue H, Wang Y-C, Nazor KL, Guo S, Trivedi N, Peterson SE, Liu Y, Loring JF, Laurent LC. 2013. “Matched miRNA and mRNA signatures from a hESC-based in vitro model of pancreatic differentiation reveal novel regulatory interactions.” Journal of Cell Science, 126:3848-3861.
Jones JC, Sabatini K, Liao X, Tran HT, Lynch CL, Morey RE, Glenn-Pratola V, Boscolo FS, Yang Q, Parast MM, Liu Y, Peterson SE, Laurent LC, Loring JF, Wang YC. “Melanocytes derived from transgene-free human induced pluripotent stem cells.” Journal of Investigative Dermatology, epub ahead of print.
Song HW, Anderson RA, Bayne RA, Gromoll J, Shimasaki S, Chang RJ, Parast MM, Laurent LC, de Rooij DG, Hsieh TC, Wilkinson MF. 2013. “The RHOX homeobox gene cluster is selectively expressed in human oocytes and male germ cells.” Human Reproduction, epub ahead of print.
Kurian L, Sancho-Martinez I, Nivet E, Moon K, Pendaries C, Volle-Challier C, Bono F, Herbert J-M, Xia Y, Li M, Pulecio J, Montserrat N, Ruiz S, Aguirre A, Dubova I, Rodriguez C, Denli AM, Boscolo FS, Thiagarajan RD, Gage FH, Loring JF, Laurent LC, Belmonte JCI.  2013. “Conversion of Human Fibroblasts Into Vascular Cells.” Nat Methods, 10(1):77-83.
Ramskold D, Luo S, Wang Y-C, Li R, Deng Q, Daniels GA, Khrebtukova I, Loring JF, Laurent LC, Schroth GP, Sandberg R. 2012. “Full-Length mRNA-Seq from Single Cell Levels of RNA and Individual Circulating Tumor Cells.” Nat Biotechnol, 30(8):777-82.
Norton ME, Brar H, Weiss J, Karimi A, Laurent LC, Caughey A, Rodriguez H, Williams J, Mitchell M, Adair CD, Lee H, Jacobbson B, Tomlinson MW, Oepkes D, Hollemon D, Sparks A, Oliphant A, Song K.  2012. “Non-Invasive Chromosomal Evaluation (NICE) Study:  Results of a Multicenter, Prospective, Cohort Study for Fetal Trisomy Detection.” Am J Obstet Gynecol, 207(2):137.e1-8.
Wang W, Feng L, Zhang H-H, Hachey S, Satohisha S, Laurent LC, Parast M, Zheng J, Chen D.  2012. “Preeclampsia upregulates angiogenesis-associated microRNAs (miR-17, 20a and 20b) that target ephrin B2 and EPHB4 in human placenta.” J Clin End and Met., 97(6):E1051-9.
Nazor KL, Altun G, Lynch C, Tran H, Harness JV, Slavin I, Garitaonandia I, Müller F-J, Wang Y-C, Boscolo FS, Fakunle E, Lee S, Park HS, Martynova M, Olee T, D'Lima DD, Semechkin R, Parast M, Galat V, Laslett AL, Schmidt U, Keirstead HS, Loring JF, Laurent LC. 2012. “Recurrent Variations in DNA Methylation in Human Pluripotent Stem Cells and their Differentiated Derivatives.”  Cell Stem Cell, 10(5):620-34.
Liao W-X, Laurent L, Agent S, Hodges J, Chen D-B.  2012.  “Human Placental Expression of SLIT/ROBO Signaling Cues: Effects of Preeclampsia and Hypoxia.” Biology of Reproduction, 86(4):111.
  Ruff D, Macarthur C, Tran H, Bergseid J, Tian J, Shannon M, Chen SM, Fontes A, Laurent L, Swartzman E, Taliana A, Rao M, Lieu P.  2012.  “Applications of Quantitative PCR Protein Assays During Reprogramming.”  Stem Cells and Development, 21(4):530-8.
MacArthur CC, Xue H, Van Hoof D, Lieu PT, Dudas M, Fontes A, Swistowski A, Touboul T, Seerke R, Laurent LC, Loring JF, German MS, Zeng X, Rao MS, Lakshmipathy U, Chesnut JD, Liu Y.  2011.  “Chromatin Insulator Elements Block Transgene Silencing in Engineered Human Embryonic Stem Cell Lines at a Defined Chromosome 13 Locus.”  Stem Cells and Development, 21(2):191-205.
Israel MA, Yuan SH, Bardy C, Reyna SM, Mu Y, Herrera C, Hefferan MP, Van Gorp S, Marsala M, Nazor KL, Boscolo FS, Laurent LC, Carson CT, Gage FH, Remes AM, Koo EH, Goldstein LSB.  2012.  “Probing sporadic and familial Alzheimer's disease using induced pluripotent stem cells.” Nature, 482:216-220.
Fan JB, Chen J, April CS, Fisher JS, Klotzle B, Bibikova M, Kaper F, Ronaghi M, Linnarsson S, Ota T, Chien J, Laurent LC, Nisperos SV, Chen GY, Zhong JF.  2012.  “Highly Parallel Genome- Wide Expression Analysis of Single Mammalian Cells.” PLoSOne, 7(2): e30794.
Peterson SE, Tran HT, Garitaonandia I, Han S, Nickey KS, Leonardo T, Laurent LC, Loring JF.  2011.  “Teratoma Generation in the Testis Capsule.”  Journal of Visualized Experiments, Issue 57.
Wang Y-C, Nakagawa M, Garitaonandia I, Slavin I, Altun G, Lacharite R, Nazor K, Tran H, Lynch C, Leonardo T, Liu Y, Peterson S, Laurent LC, Yamanaka S, Loring JF.  2011.  “Specific lectin biomarkers for isolation of human pluripotent stem cells identified through array-based glycomic analysis.”  Cell Research, 21(11):1551-63.
Ben-Nun IF, Montague SC, Houck ML, Tran HT, Baritaonandia I, Leonardo TR, Wang Y-C, Charter SJ, Laurent LC, Ryder OA, Loring JF.  2011.  “Generation of induced pluripotent stem cells from highly endangered species.”  Nature Methods, 8(10):829-31.
Golob J, Kumar R, Guenther M, Pabon L, Pratt GA, Loring JF, Laurent LC, Young R, Murry CE.  2011. “Evidence That Gene Activation and Silencing During Stem Cell Differentiation Requires a Transcriptionally Paused Intermediate State.” PLoS ONE, 6(8):e22416.
Peterson SE, Westra JW, Rehen SK, Young H, Buschman D, Paczkowski CM, Yung YC, Lynch CL, Tran HT, Nickey KS, Wang Y-C, Laurent LC, Loring JF, Carpenter MK, Chun J. 2011.  “Normal human pluripotent stem cell lines exhibit pervasive mosaic aneuploidy.”  PLoS One, 6(8):e23018.
Liu G-H, Suzuki K, Qu J, Sancho-Martinez I, Yi F, Li M, Kumar S, Nivet E, Kim J, Soligalla D, Dubova I, Goebl A, Plongthongkum N, Fung H-L, Zhang K, Loring JF, Laurent LC, Belmonte JCI.  2011.  “Targeted Gene Correction of Laminopathy-Associated LMNA Mutations in Patient-Specific iPSCs.”  Cell Stem Cell, 8(6):688-94.
Muller FJ, Schuldt BM, Williams R, Mason D, Altun G, Papapetrou E, Danner S, Goldman JE, Herbst A, Schmidt NO,  Aldenhoff JB, Laurent LC, Loring JF.  2011.  “A bioinformatic open access assay for pluripotency in human cells.”  Nature Methods, 8(4):315-17.
Harness JV, Turovets NA, Seiler MJ, Nistor G, Altun G, Agapova LS, Ferguson D, Laurent LC, Loring JF, Keirstead HS.  2011.  “Equivalence of Conventionally-Derived and Parthenote-Derived Human Embryonic Stem Cells.”  PlosOne.  6(1):e14499.
Laurent LC, Ulitsky I, Slavin I, Tran H, Schork A, Morey R, Lynch C, Harness JV, Lee S, Barrero MJ, Ku S, Martynova M, Semechkin R, Galat V, Gottesfeld J, Belmonte JCI, Murry C, Keirstead HS, Park H-S, Schmidt U, Laslett AL, Muller F-J, Nievergelt CM, Shamir R, Loring JF. 2011.  “Dynamic Changes in the Copy Number of Pluripotency and Cell Proliferation Genes in Human ES and iPS Cells during Reprogramming and Time in Culture.”  Cell Stem Cell.  8(1):106-118.
Ku S, Soragni E, Campau E, Thomas EA, Altun G, Laurent L, Loring JF, Napierala M, Gottesfeld JM. 2010.  “Friedreich’s Ataxia Induced Pluripotent Stem Cells Recapitulate GAA•TTC Triplet-Repeat Instability.”  Cell Stem Cell. 7(5):631-7.
Ulitsky I, Laurent LC, Shamir R.  2010.  "Towards Computational Prediction of  MicroRNA Function and Activity." Nucleic Acids Research. 38(15):e160.
Laurent LC, Wong E, Li GL, Huynh T, Tsirigos A, Li R, Zhou G, Ong CT, Low HM, Sun W-K, Rigoutsos I, Wang J, Loring JF, Wei C-L.  2010.  "Dynamic Changes in the Human Methylome During Differentiation."  Genome Research.  20(3):320-31.
Abraham S, Sheridan SD, Laurent LC, Albert K, Stubban C, Ulitsky I, Miller B, Loring JF, Rao R.  2010.  "Propagation of human embryonic and induced pluripotent stem cells in an indirect co-culture system." Biochem Biophys Res Commun.  393(2):211-6.
Laurent LC, Nievergelt CM, Lynch C, Fakunle E, Harness J, Schmidt U, Galat V, Laslett AL, Otonkoski T, Keirstead H, Schork A, Park H-S, Loring JF.  2010.  "Restricted Ethnic Diversity in Human Embryonic Stem Cells." Nature Methods.  7(1):6-7.
Muller F-J, Laurent LC, Kostka D, Ulitsky I, Williams R, Lu C, Rao MS, Shamir R, Schwartz PH, Schmidt NO, Loring JF.  2008.  “Regulatory networks define phenotypic classes of human stem cell lines.” Nature.  455:401-405. 
Laurent LC, Chen J, Ulitsky I, Mueller F-J, Lu C, Shamir R, Fan J-B, Loring JF.  2008.                        “Comprehensive MicroRNA profiling reveals a unique human embryonic stem cell signature dominated by a single seed sequence.”  Stem Cells, 26:1506-1516.
Rothenberg SM, Olsen MN, Laurent LC, Crowley RA, Brown PO. 2001.  “Comprehensive mutational analysis of the Moloney murine leukemia virus envelope protein.” Journal of Virology , 75(23):11851-62.
Laurent LC, Olsen MN, Crowley RA, Savilahti H, Brown PO.  2000.  “Functional Characterization of the HIV-1 Genome by Genetic Footprinting.”  Journal of Virology, 74(6):2760-2769.
Lodi PJ; Chang LC; Knowles JR; Komives EA.  1994.  "Triosephosphate isomerase requires a positively charged active site: the role of lysine-12."  Biochemistry.  33(10):2809-14.
Perry KM, Carreras CW, Chang LC, Santi DV, Stroud RM.  1993.  "Structures of thymidylate synthase with a C-terminal deletion:  role of the C-terminus in alignment of 2'-deoxyuridine 5'- monophosphate and 5,10-methylenetetrahydrofolate."  Biochemistry.  32(28):7116-25.
  Patwardhan S, Gashler A, Siegel MG, Chang LC, Joseph LJ, Shows TB, Le Beau MM, Sukhatme VP.  1991.  "EGR3, a novel member of  the Egr family of genes encoding immediate early transcription factors."  Oncogene.  6(6):917-28.
  Komives EA, Chang LC, Lolis E, Tilton RF, Petsko GA, Knowles JR.  1991.  "Electrophilic catalysis in triosephosphate isomerase:  the role of histidine-95."  Biochemistry.  30(12):3011-9.
  Sukhatme VP, Cao X, Chang LC, Tsai-Morris C-H, Stamenkovich D, Ferreira PCP, Cohen DR, Edwards SA, Shows TB, Curran T, Le Beau MM, Adamson ED.  1988.  "A zinc finger-encoding gene coregulated with c-fos during growth and differentiation, and after cellular depolarization."  Cell.  53:37-43.
Joseph LJ, Chang LC, Stamenkovich D, Sukhatme VP.  1988.  "Complete nucleotide and deduced amino acid sequences of human and murine preprocathepsin L, an abundant transcript induced by transformation of fibroblasts."  Journal of Clinical Investigation.  81:1621-1629.

 

Invited Articles and Reviews

Thiagarajan RD, Morey R, Laurent LC. 2014. “The epigenome in pluripotency and differentiation.”  Epigenomics, 6(1):121-37.
Morey R, Laurent LC. 2014. “Getting off the ground state: X chromosome inactivation knocks down barriers to differentiation.” Preview article, Cell Stem Cell, 14:131-2.
Leonardo TR, Schultheisz HL, Loring JF, Laurent LC. 2012.  “The functions of microRNAs in             pluripotency and reprogramming.” Nature Cell Biology, 14(11):1114-21.
Nazor KL, Loring JF, Laurent LC.  2012.  “Equally potent? Does cellular reprogramming justify the     abandonment of human embryonic stem cells?.” EMBO Reports, 13(10)890-4.
  Altun G, Laurent LC, Loring JF.  "Epigenetic Remodeling and Stem Cells."  Drug Discovery Today,     2010 April 22:5(4):139-42.
  Altun G, Loring JF, Laurent LC.  2009.  "DNA Methylation in Embryonic Stem Cells."  Journal of Cellular Biochemistry, 109(1):1-6.
Laurent LC.  2008. "MicroRNAs in Embryonic Stem Cells and Early Embryonic Development.”  Journal of Cellular and Molecular Medicine, 12(6A):2181-2188.
Bibikova M, Laurent LC, Ren B, Loring JF, Fan J-B.  2008.  “Unraveling the epigenome of embryonic stem cells.”  Cell Stem Cell, 2(2):123-34.

 

Books and Book Chapters

Bibikova M, Nazor KL, Altun G, Laurent LC.  2012.  “Epigenetics:  DNA Methylation.”  In Loring JF and Peterson SE, Human Stem Cell Manual (2nd Edition), pp. 325-336, Elsevier.

Nazor KL, Thiagarajan RD, Laurent LC. 2012.  “Analysis of Genome-Wide Gene Expression Data from Microarrays and Sequencing.”  In Loring JF and Peterson SE, Human Stem Cell Manual (2nd Edition), pp. 271-291, Elsevier.

Boscolo F, Lynch CL, Morey RE, Laurent LC.  2012.  “SNP Genotyping to Detect Genomic Alterations in Human Pluripotent Stem Cells.”  In Loring JF and Peterson SE, Human Stem Cell Manual (2nd Edition), pp. 203-221, Elsevier.
Rajan P, Smotrich D, Ross R, Laurent LC, Loring JF.  2007.  “Derivation of Embryonic Stem Cells from Human Blastocysts.”  In Loring JF, Wesselschmidt RL, and Schwartz PH, Human Stem Cell Manual, pp. 291-308, Elsevier.

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